Mesocestoides corti: a LIM-homeobox gene upregulated during strobilar



Lalanne AI, Britos L, Ehrlich R, Castillo E.


Seccion Bioquimica, Facultad de Ciencias, Igua 4225, CP 11400 Montevideo,



To understand the molecular processes regulating morphological changes during

cestode life histories we focused on homeodomain (HD) proteins, a family of

transcription factors essential for pattern formation during development. In

this study we report the isolation of the partial sequence of MvLim, a LIM-HD

gene of Mesocestoides corti. Other members of this gene family, characterized in

Drosophila melanogaster, Caenorhabditis elegans and vertebrates contribute to

cell fate determination of various neuronal subtypes. Phylogenetic analyses

showed that MvLim clusters with members of the LIN-11 group and that

platyhelminths have at least two different LIM-HD genes. By real time PCR we

determined that MvLim expression is 20-fold greater in segmented worms than in

tetrathyridia. The enhancement of MvLim expression during strobilation could be

associated to changes in the innervation pattern occurring in proglottids